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Product Name
SMYD2 antibody
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Description
SMYD2 Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive IHC detected in human heart tissue. Positive WB detected in mouse skeletal muscle tissue, HepG2 cells, Jurkat cells, mouse heart tissue. Observed molecular weight by Western-blot: 50kd
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
HSKM B antibody; KMT3C antibody; Lysine N methyltransferase 3C antibody; SMYD2 antibody; ZMYND14 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SMYD2 recombinant protein (Accession Number: XM_047425702). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:115407(SMYD2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human heart using Catalog No:115407(SMYD2 antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of Hela cells, using SMYD2 antibody Catalog No:115407 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
SMYD2 represents SET and MYND domain containing 2
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"