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Product Name
SMU1 antibody
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Description
SMU1 Rabbit Polyclonal antibody. Positive WB detected in mouse liver tissue, K-562 cells. Positive IHC detected in human kidney tissue, human brain tissue, human hepat℃irrhosis tissue, human ovary tissue, human testis tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 45-50 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BWD antibody; DKFZp761L0916 antibody; FLJ10805 antibody; FLJ10870 antibody; FLJ11970 antibody; fSAP57 antibody; RP11 54K16.3 antibody; SMU 1 antibody; SMU1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SMU1 recombinant protein (Accession Number: NM_018225). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:115404(SMU1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human kidney using Catalog No:115404(SMU1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:115404(SMU1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using SMU1 antibody Catalog No:115404 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
Sum1, belongs to the WD repeat SMU1 family. Human SMU1 is a component of the spliceosome fSAP57(PMID: 15254247). It is possible that the mutation in Smu1 affects spliceosome function, resulting in generation of improper splice variants, which could explain the phenotypes of tsTM18 cells. Some results suggest that Smu1 participates in at least two steps of splicing: splice-site choice and control of accuracy(PMID: 17105761). This antibody is a rabbit polyclonal antibody raised against a region of human REDD1 antigen.
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