-
Product Name
SMARCD3 antibody
- Documents
-
Description
SMARCD3 Rabbit Polyclonal antibody. Positive WB detected in BxPC-3 cells, HeLa cells. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 54kd
-
Tested applications
ELISA, WB, IF
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
BAF60C antibody; BRG1 assOCiated factor 60C antibody; CRACD3 antibody; Rsc6p antibody; SMARCD3 antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of SMARCD3 recombinant protein (Accession Number: NM_003078). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:20-1:200
-
Validations
BxPC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:115435(SMARCD3 antibody) at dilution of 1:400
Immunofluorescent analysis of Hela cells, using SMARCD3 antibody Catalog No:115435 at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).
-
Background
SMARCD3 is a component of the SWI/SNF (mating type switching/sucrose non-fermenting) chromatin remodeling complex, which evolutionarily conserved from yeast to human, and all complexes contain a core set of conserved components, including BRG1/Brm-associated factors (BAFs) and a DNA-dependent SWI2/SNF2-like ATPase, which enables chromatin remodeling. SMARCD3 isoforms bind to several nuclear receptors and transcription factors of various families. SMARCD3 proteins interact in a ligand-independent manner with peroxisome proliferator- activated receptor gamma and enhance its transcriptional activity.
-
References
- D'Aniello C, Fiorenzano A, Iaconis S. The G-protein-coupled receptor APJ is expressed in the second heart field and regulates Cerberus-Baf60c axis in embryonic stem cell cardiomyogenesis. Cardiovascular research. 100(1):95-104. 2013.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"