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Product Name
SMARCD1 antibody
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Description
SMARCD1 Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, human brain tissue, Jurkat cells. Positive IP detected in mouse brain tissue. Positive IHC detected in human lymphoma tissue. Positive IF detected in SH-SY5Y cells. Observed molecular weight by Western-blot: 58 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BAF60A antibody; BRG1 assOCiated factor 60A antibody; CRACD1 antibody; Rsc6p antibody; SMARCD1 antibody; SWI/SNF complex 60 kDa subunit antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SMARCD1 recombinant protein (Accession Number: BC009368). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:115433(SMARCD1 antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:115433(SMARCD1 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of SH-SY5Y cells, using SMARCD1 antibody Catalog No:115433 at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-SMARCD1 (IP:Catalog No:115433, 3ug; Detection:Catalog No:115433 1:600) with mouse brain tissue lysate 4000ug.
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Background
SMARCD1 belongs to the nuclear SWI/SNF chromatin remodeling complex, which regulates transcription and interacts with nucleolin in the nucleus. In addition, it's a member of the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). It possesses a SWIB domain which is homologous to and shares a common fold with the p53-binding domain of MDM2, a cell cycle regulator which interacts with NPM1. SMARCD1 has a strong influence on the Vitamin D-mediated transcriptional activity from an enhancer Vitamin D receptor element (VDRE). And it can mediates critical interactions between nuclear receptors and the BRG1/SMARCA4 chromatin-remodeling complex for transactivation.
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References
- Oya H, Yokoyama A, Yamaoka I. Phosphorylation of Williams syndrome transcription factor by MAPK induces a switching between two distinct chromatin remodeling complexes. The Journal of biological chemistry. 284(47):32472-82. 2009.
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