Cart 2

SMARCB1 antibody

Cat.#: 115431

Size:

Special Price 239.0 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    SMARCB1 antibody
  • Documents
  • Description
    SMARCB1 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, K-562 cells. Positive IP detected in K-562 cells. Positive IHC detected in human lymphoma tissue, human prostate cancer tissue. Observed molecular weight by Western-blot: 40-44 kDa
  • Tested applications
    ELISA, WB, IP, IHC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    BAF47 antibody; BRG1 assOCiated factor 47 antibody; hSNF5 antibody; hSNFS antibody; INI1 antibody; Integrase interactor 1 protein antibody; RDT antibody; Sfh1p antibody; SMARCB1 antibody; SNF5 antibody; SNF5 homolog antibody; SNF5L1 antibody; Snr1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Peptide (Accession Number: NM_003073). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:115431(SMARCB1 antibody) at dilution of 1:500

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:115431(SMARCB1 antibody) at dilution of 1:500

    IP Result of anti-SMARCB1 (IP:Catalog No:115431, 4ug; Detection:Catalog No:115431 1:500) with K-562 cells lysate 3200ug.

    IP Result of anti-SMARCB1 (IP:Catalog No:115431, 4ug; Detection:Catalog No:115431 1:500) with K-562 cells lysate 3200ug.

    Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using Catalog No:115431(SMARCB1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using Catalog No:115431(SMARCB1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

  • Background
    SMARCB1, also named as BAF47, INI1 and SNF5L1, belongs to the SNF5 family. It is a core component of the BAF (hSWI/SNF) complex. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. SMARCB1 stimulates in vitro the remodeling activity of SMARCA4/BRG1/BAF190A. It is involved in activation of CSF1 promoter. SMARCB1 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. SMARCB1 plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Defects in SMARCB1 are a cause of rhabdoid tumor (RDT) which also known as malignant rhabdoid tumor (MRT). Defects in SMARCB1 are a cause of schwannomatosis. The antibody is specific to SMARCB1.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"