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Product Name
SMARCA4 antibody
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Description
SMARCA4 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HepG2 cells, human placenta tissue, mouse brain tissue, rat brain tissue. Positive IP detected in HeLa cells. Positive IHC detected in human lung cancer tissue, human gliomas tissue, human lung cancer slides. Positive IF detected in HepG2 cells, HEK-293 cells, Hela cells. Observed molecular weight by Western-blot: 185kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ATP dependent helicase SMARCA4 antibody; BAF190 antibody; BAF190A antibody; BRG1 antibody; BRG1 assOCiated factor 190A antibody; FLJ39786 antibody; hSNF2b antibody; Protein brahma homolog 1 antibody; Protein BRG 1 antibody; SMARCA4 antibody; SNF2 antibody; SNF2 BETA antibody; SNF2B antibody; SNF2L4 antibody; SNF2LB antibody; SWI2 antibody; Transcription activator BRG1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SMARCA4 recombinant protein (Accession Number: NM_001128844). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115427(SMARCA4 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:115427(SMARCA4 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using SMARCA4 antibody Catalog No:115427 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-SMARCA4 (IP:Catalog No:115427, 5ug; Detection:Catalog No:115427 1:1000) with HeLa cells lysate 2500ug.
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Background
SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
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References
- Araujo LH, Timmers C, Bell EH. Genomic Characterization of Non-Small-Cell Lung Cancer in African Americans by Targeted Massively Parallel Sequencing. Journal of clinical oncology : official journal of the American Society of Clinical Oncology. 33(17):1966-73. 2015.
- Wang Y, He L, Du Y. The long noncoding RNA lncTCF7 promotes self-renewal of human liver cancer stem cells through activation of Wnt signaling. Cell stem cell. 16(4):413-25. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"