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Product Name
Anti-BRG1 (4E11) Mouse antibody
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Description
BRG1 (4E11) Mouse monoclonal antibody
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Tested applications
WB, IP
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Species reactivity
Human
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Alternative names
ATP dependent helicase SMARCA4;ATP-dependent helicase SMARCA4;BAF 190;BAF190;BAF190A; Brahma protein homolog 1;Brahma protein like 1;BRG1;BRG1 associated factor 190A;BRG1 protein;BRG1-associated factor 190A;BRM/SWI2 related gene 1;Global transcription act antibody
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant human BRG1 protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
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Validations
Western blot detection of SBRG1 in Hela NE and K562 cell lysates using BRG1 mouse mAb (1:1000 diluted).Predicted band size:220KDa.Observed band size:220KDa.
Immunoprecipitation analysis of Hela cells lysates. IP: BRG1 mouse mAb(168211); WB: BRG1 mouse mAb(201025).
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Background
Swiss-Prot Acc.P51532.ATP-dependent chromatin remodeling complexes play an essential role in the regulation of various nuclear processes, such as gene expression, DNA replication, and repair. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"