-
Product Name
SMAD9 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to SMAD9
-
Tested applications
WB, IHC, IF
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
SMAD9 antibody; MADH6 antibody; MADH9 antibody; PPH2 antibody; SMAD8 antibody; SMAD8/9 antibody; SMAD8A antibody; SMAD8B antibody; SMAD family member 9 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SMAD9 (NP_005896.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - SMAD9 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SMAD9 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunohistochemistry - SMAD9 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver injury using SMAD9 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SMAD9 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human brain cancer using SMAD9 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SMAD9 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human kidney using SMAD9 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SMAD9 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human kidney cancer using SMAD9 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SMAD9 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using SMAD9 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SMAD9 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse kidney using SMAD9 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - SMAD9 Polyclonal Antibody
Immunofluorescence analysis of A549 cells using SMAD9 antibody . Blue: DAPI for nuclear staining.
-
Background
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"