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SMAD5 antibody

Cat.#: 115421

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Product Information

  • Product Name
    SMAD5 antibody
  • Documents
  • Description
    SMAD5 Rabbit Polyclonal antibody. Positive FC detected in MCF-7 cells. Positive IF detected in MCF-7 cells. Positive WB detected in K-562 cells. Positive IP detected in K-562 cells. Observed molecular weight by Western-blot: 52 kDa
  • Tested applications
    ELISA, IF, WB, FC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    DKFZp781C1895 antibody; DKFZp781O1323 antibody; Dwfc antibody; hSmad5 antibody; JV5 1 antibody; MAD homolog 5 antibody; MADH5 antibody; Mothers against DPP homolog 5 antibody; SMAD 5 antibody; SMAD family member 5 antibody; SMAD5 antibody
  • Immunogen
    Human SMAD5 Recombinant protein (GST tag)
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of SMAD5 recombinant protein (Accession Number: XM_017009470). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IF: 1:10-1:100

  • Validations

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:115421(SMAD5 Antibody) at dilution of 1:1000

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:115421(SMAD5 Antibody) at dilution of 1:1000

    Immunofluorescent analysis of MCF-7 cells, using SMAD5 antibody Catalog No:115421 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of MCF-7 cells, using SMAD5 antibody Catalog No:115421 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    IP Result of anti-SMAD5 (IP:Catalog No:115421, 4ug; Detection:Catalog No:115421 1:1000) with K-562 cells lysate 3200ug.

    IP Result of anti-SMAD5 (IP:Catalog No:115421, 4ug; Detection:Catalog No:115421 1:1000) with K-562 cells lysate 3200ug.

    1X10^6 MCF-7 cells were stained with 0.2ug SMAD5 antibody (Catalog No:115421, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 MCF-7 cells were stained with 0.2ug SMAD5 antibody (Catalog No:115421, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    SMAD5, also named as MADH5 and JV5-1, is a transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). It plays a critical role in the signaling pathway by which TGF-beta inhibits the proliferation of human hematopoietic progenitor cells.
  • References
    • Zhang XY, Shen BR, Zhang YC. Induction of thoracic aortic remodeling by endothelial-specific deletion of microRNA-21 in mice. PloS one. 8(3):e59002. 2013.
    • Yao QP, Zhang P, Qi YX. The role of SIRT6 in the differentiation of vascular smooth muscle cells in response to cyclic strain. The international journal of biochemistry & cell biology. 49:98-104. 2014.
    • Zeng Y, Liu H, Kang K. Hypoxia inducible factor-1 mediates expression of miR-322: potential role in proliferation and migration of pulmonary arterial smooth muscle cells. Scientific reports. 5:12098. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"