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Product Name
Anti-SMAD5 (4B10) Mouse antibody
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Description
SMAD5 (4B10) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF, FC
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Species reactivity
Human
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Alternative names
DKFZp781C1895;DKFZp781O1323;Dwfc;hSmad 5;hSmad5;JV5 1;JV5-1;MAD homolog 5;MAD mothers against decapentaplegic homolog 5;MAD antibody; mothers against decapentaplegic homolog 5;MADH 5;MADH5;Mothers against decapentaplegic homolog 5;Mothers against DPP homolog 5;Mus antibody
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant human SMAD5 (C-terminus) protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
1/75;FC: 1/100
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Validations
Western blot detection of SMAD5 (C-terminus) in Hela,Jurkat and K562 cell lysates using SMAD5 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size:52KDa.Observed band size:60KDa.
Flow Cytometry analysis of Jurkat cells stained with SMAD5 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG.
Immunocytochemistry of HeLa cells using anti-SMAD5 (C-terminus) mouse mAb diluted 1:75.
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Background
Swiss-Prot Acc.Q99717.Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
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