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Product Name
SMAD1 antibody
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Description
SMAD1 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells, HepG2 cells. Positive WB detected in HepG2 cells, HeLa cells, mouse heart tissue. Positive IP detected in mouse heart tissue. Observed molecular weight by Western-blot: 52 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BSP 1 antibody; BSP1 antibody; hSMAD1 antibody; JV4 1 antibody; JV41 antibody; MAD homolog 1 antibody; Mad related protein 1 antibody; MADH1 antibody; MADR1 antibody; Mothers against DPP homolog 1 antibody; SMAD 1 antibody; SMAD family member 1 antibody; SMAD1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SMAD1 recombinant protein (Accession Number: NM_001354813). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:115415(SMAD1 antibody) at dilution of 1:300
IP Result of anti-SMAD1 (IP:Catalog No:115415, 4ug; Detection:Catalog No:115415 1:500) with mouse heart tissue lysate 8000ug.
Immunofluorescent analysis of HeLa cells using Catalog No:115415(SMAD1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Transforming growth factor-β (TGF-β) superfamily is recognized as one of the largest families of secreted multifunctional peptides exerting different biological effects on a large variety of cell types, such as regulation of hormone secretion, stimulation of extracellular matrix formation, the inhibition of proliferation of many cell types, cell survival, bone formation, and chemotaxis for inflammatory cells. One of the most important proteins that modulate TGF-β ligand activity is the SMAD family proteins. SMAD1 is one of the receptor-activated Smads. It's also a signal transducers of BMP signaling and binds to several proteins involved in ubiquitin-proteasome system (UPS). Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase
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References
- Dong Z, Lu X, Yang Y. IL-27 alleviates the bleomycin-induced pulmonary fibrosis by regulating the Th17 cell differentiation. BMC pulmonary medicine. 15:13. 2015.
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