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Product Name
SKP1 antibody
- Documents
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Description
SKP1 Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue. Positive IF detected in HepG2 cells. Positive WB detected in human brain tissue, HeLa cells. Observed molecular weight by Western-blot: 19 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
EMC19 antibody; NET19 antibody; OCP 2 antibody; OCP II antibody; OCP2 antibody; Organ of Corti protein 2 antibody; Organ of Corti protein II antibody; p19A antibody; p19skp1 antibody; SIII antibody; SKP1 antibody; SKP1A antibody; TCEB1L antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SKP1 recombinant protein (Accession Number: NM_170679). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:115289(SKP1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:115289(SKP1 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using SKP1 antibody Catalog No:115289 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
SKP1(S-phase kinase-associated protein 1) is also named as EMC19, ocP2, SKP1A, TCEB1L,belongs to the SKP1 family.It is an essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"