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Anti-Skp1 (4G3) Mouse antibody

Cat.#: 168495

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Product Information

  • Product Name
    Anti-Skp1 (4G3) Mouse antibody
  • Documents
  • Description
    Skp1 (4G3) Mouse monoclonal antibody
  • Tested applications
    WB, IHC-P, FC
  • Species reactivity
    Human
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Purified recombinant fragment of human SKP1 expressed in E. Coli.
  • Clonality
    Monoclonal
  • Formulation
    Ascitic fluid containing 0.03% sodium azide.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/500 - 1/2000

    IHC: 1/200 - 1/1000

    FC: 1/200 - 1/400

    ELISA: 1/10000

  • Validations

    Western blot analysis using SKP1 mouse mAb against Hela (1), RAJI (2), Jurkat (3), MCF-7 (4), HepG2 (5), PC-12 (6) and Cos7 (7) cell lysate.

    Western blot analysis using SKP1 mouse mAb against Hela (1), RAJI (2), Jurkat (3), MCF-7 (4), HepG2 (5), PC-12 (6) and Cos7 (7) cell lysate.

    Western blot detection of Skp1 in Jurkat,A549,MCF7,C6 and Hela cell lysates using Skp1 mouse mAb (1:5000 diluted).Predicted band size:19KDa.Observed band size:19KDa.

    Western blot detection of Skp1 in Jurkat,A549,MCF7,C6 and Hela cell lysates using Skp1 mouse mAb (1:5000 diluted).Predicted band size:19KDa.Observed band size:19KDa.

    Flow cytometric analysis of Hela cells using SKP1 mouse mAb (green) and negative control (red).

    Flow cytometric analysis of Hela cells using SKP1 mouse mAb (green) and negative control (red).

    Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using SKP1 mouse mAb with DAB staining.

    Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using SKP1 mouse mAb with DAB staining.

    Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using SKP1 mouse mAb with DAB staining.

    Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using SKP1 mouse mAb with DAB staining.

  • Background
    Swiss-Prot Acc.P63208.This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"