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Product Name
SIRT1 antibody
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Description
SIRT1 Mouse Monoclonal antibody. Positive IHC detected in human testis tissue. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, K-562 cells. Observed molecular weight by Western-blot: 110-130 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
hSIR2 antibody; hSIRT1 antibody; SIR2 like protein 1 antibody; SIR2L1 antibody; SIRT1 antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of SIRT1 recombinant protein (Accession Number: BC012499). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107587(SIRT1 Antibody) at dilution of 1:1000
IP Result of anti-SIRT1 (IP:Catalog No:107587, 5ug; Detection:Catalog No:107587 1:1000) with HeLa cells lysate 2000ug.
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:107587(SIRT1 Antibody) at dilution of 1:50 (under 40x lens)
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Background
SIRT1, also named as SIR2L1, contains a deacetylase sirtuin-type domain and belongs to the sirtuin family. It regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. SIRT1 in particular initiates several signalling events relevant to cardioprotection, including: activation of endothelial nitric oxide synthase, insulin receptor signalling, and autophagy. In addition SIRT1 activation elicits resistance to oxidative stress via regulation of transcription factors and co-activators such as FOXO, Hif-2a, and NF-kB. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"