SIL1 antibody

Cat.#: 115232

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Product Information

  • Product Name
    SIL1 antibody
  • Documents
  • Description
    SIL1 Rabbit Polyclonal antibody. Positive WB detected in L02 cells, mouse kidney tissue, mouse liver tissue. Positive IHC detected in human kidney tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 52 kDa
  • Tested applications
    ELISA, WB, IF, IHC
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    BAP antibody; BiP assOCiated protein antibody; MSS antibody; SIL1 antibody; ULG5 antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of SIL1 recombinant protein (Accession Number: NM_022464). Purification method: Antigen Affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:115232(SIL1 antibody) at dilution of 1:1500

    L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:115232(SIL1 antibody) at dilution of 1:1500

    Immunofluorescent analysis of HepG2 cells using Catalog No:115232(SIL1 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

    Immunofluorescent analysis of HepG2 cells using Catalog No:115232(SIL1 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

    Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:115232(SIL1 Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:115232(SIL1 Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:115232(SIL1 Antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:115232(SIL1 Antibody) at dilution of 1:50 (under 40x lens)

  • Background
    SIL1 represents SIL1 homolog, endoplasmic reticulum chaperone (S. cerevisiae)
  • References
    • Ichhaporia VP, Sanford T, Howes J, Marion TN, Hendershot LM. Sil1, a nucleotide exchange factor for BiP, is not required for antibody assembly or secretion. Molecular biology of the cell. 26(3):420-9. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"