Shepherin II peptide
Not For Human Use, Lab Use Only.
Cat.#: 301647
Optional Service:
TFA RemovalWhat's this?
Special Price 480.7 USD
Availability:
4 weeks
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Product Name
Shepherin II peptide
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Documents
Batch to batch variation of the purity
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Sequence Shortening
GYHGGHGGHGGGYNGGGGHGGHGGGYNGGGHHGGGGHG
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Sequence
H-Gly-Tyr-His-Gly-Gly-His-Gly-Gly-His-Gly-Gly-Gly-Tyr-Asn-Gly-Gly-Gly-Gly-His-Gly-Gly-His-Gly-Gly-Gly-Tyr-Asn-Gly-Gly-Gly-His-His-Gly-Gly-Gly-Gly-His-Gly-OH
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Length (aa)
38
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Peptide Purity (HPLC)
97.1%
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Molecular Formula
C133H172N56O44
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Molecular Weight
3259.1
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Source
Synthetic
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Form
Powder
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Description
Shepherin I and Shepherin II are two unique gly-rich AMPs (~65%) generated from the same precursor protein using different polypeptide regions. Due to multiple His residues, their net charge depend on pH. IN TFE, no ordered structure was found by CD. The original has been replaced. Provided by Biswajit Mishra. 9/6/2012. Shepherin II has Antibacterial, Antifungal activity. Shepherin II was found in roots, shepherd's purse, Capsella bursa-pastoris.
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Storage Guidelines
Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides
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References
- Park CJ, Park CB, Hong SS, Lee HS, Lee SY, Kim SC. Characterization and cDNA cloning of two glycine- and histidine-rich antimicrobial peptides from the roots of shepherd's purse, Capsella bursa-pastoris. Plant Mol Biol. 2000;44(2):187-97.
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About TFA salt
Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.
TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.
TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.
In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.
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Molar Concentration Calculator
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Dilution Calculator
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Percent Concentration Calculator
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Peptide Property
- Analysed Sequence:H-GYHGGHGGHGGGYNGGGGHGGHGGGYNGGGHHGGGGHG-OH
- Chemical Formula:C133H172N56O44
- Sequence length:38
- Extinction coefficient:3840 M-1cm-1
- GRAVY:-1.22
- Mw average:3259.12
- Theoretical pI:8.09
- Data Source:Peptide Property Calculator
GRAVY = grand average of hydropathy
X: Hydrophobic uncharged residues, like F I L M V W A and P
X: Basic residues, like R K H
X: Acidic residues, like D E
X: Polar uncharged residues, like G S T C N Q and Y
Related Products / Services
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• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"