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Product Name
SHE antibody
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Description
SHE Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human breast cancer tissue, human testis tissue. Positive WB detected in mouse testis tissue. Observed molecular weight by Western-blot: 55 kDa
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Tested applications
ELISA, IHC, WB, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
DKFZp451D1511 antibody; DKFZp686E14106 antibody; RP11 350G8.8 antibody; SHE antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_001010846). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:115271(SHE Antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:115271(SHE Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:115271(SHE Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:115271(SHE Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
SHE, also known as SH2 domain-containing adapter protein E, is a 495 amino acid protein whose molecular weight is 54 kDa. The function of SHE remains unknown. This antibody specifically recognises the 54 kDa protein.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"