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Product Name
SGCE antibody
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Description
SGCE Rabbit Polyclonal antibody. Positive IP detected in mouse heart tissue. Positive WB detected in mouse brain tissue, human brain tissue, human heart tissue, mouse heart tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 50 kDa
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Tested applications
ELISA, IF, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DYT11 antibody; Epsilon sarcoglycan antibody; Epsilon SG antibody; ESG antibody; sarcoglycan antibody; epsilon antibody; SGCE antibody; ε-sarcoglycan antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SGCE recombinant protein (Accession Number: NM_003919). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:115162(SGCE antibody) at dilution of 1:500
IP Result of anti-SGCE (IP:Catalog No:115162, 4ug; Detection:Catalog No:115162 1:500) with mouse heart tissue lysate 4000ug.
Immunofluorescent analysis of HeLa cells using Catalog No:115162(SGCE Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
SGCE represents sarcoglycan, epsilon
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References
- Yokoi F, Yang G, Li J, DeAndrade MP, Zhou T, Li Y. Earlier onset of motor deficits in mice with double mutations in Dyt1 and Sgce. Journal of biochemistry. 148(4):459-66. 2010.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"