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Product Name
SERPINE2 antibody
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Description
SERPINE2 Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue, human pancreas cancer tissue. Positive WB detected in C6 cells, A549 cells, MCF7 cells, rat brain tissue, rat heart tissue. Positive IP detected in A549 cells. Observed molecular weight by Western-blot: 44-50 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
GDN antibody; Glia derived nexin antibody; Peptidase inhibitor 7 antibody; PI 7 antibody; PI7 antibody; PN 1 antibody; PN1 antibody; PNI antibody; Protease nexin 1 antibody; Protease nexin I antibody; Serpin E2 antibody; SERPINE2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SERPINE2 recombinant protein (Accession Number: XM_017004330). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
C6 cells were subjected to SDS PAGE followed by western blot with Catalog No:115208(SERPINE2 Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human testis slide using Catalog No:115208(SERPINE2 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human testis slide using Catalog No:115208(SERPINE2 Antibody) at dilution of 1:50
IP Result of anti-SERPINE2 (IP:Catalog No:115208, 3ug; Detection:Catalog No:115208 1:300) with A549 cells lysate 2800ug.
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Background
SERPINE2 also called protease nexin-1 or glial-derived neurite promoting factor, is a member of the serine protease inhibitor (SERPIN) superfamily. It would be a potent inhibitor of the urokinase-plasminogen activator (uPA), tissue-type PA (tPA), thrombin, trypsin, factor Xia, and prostasin. SERPINE2 is widely expressed in various tissues, including endothelial cells, fibroblasts, smooth muscle cells, tumor cells, glial cells, neurons, and placental cells.
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References
- Crispi S, Piccolo MT, D'Avino A. Transcriptional profiling of endometriosis tissues identifies genes related to organogenesis defects. Journal of cellular physiology. 228(9):1927-34. 2013.
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