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Product Name
SEC31A antibody
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Description
SEC31A Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, Jurkat cells. Positive IP detected in HeLa cells. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 133 kDa
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Tested applications
ELISA, WB, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ABP125 antibody; ABP130 antibody; DKFZp686N07171 antibody; HSPC275 antibody; HSPC334 antibody; KIAA0905 antibody; SEC31 like protein 1 antibody; SEC31 related protein A antibody; SEC31A antibody; SEC31L1 antibody; Web1 like protein antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SEC31A recombinant protein (Accession Number: NM_001077206). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115081(SEC31A antibody) at dilution of 1:500
IP Result of anti-SEC31A (IP:Catalog No:115081, 4ug; Detection:Catalog No:115081 1:500) with HeLa cells lysate 1080ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:115081(SEC31A Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec31 subunit of the coat complex has been shown to be essential for vesicle formation and ER-Golgi transport (PMID: 10788476). Two mammalian homologues of yeast sec31p, Sec31A and Sec31B, share about 40% amino acid identity. Sec31A transcripts are ubiquitously and abundantly expressed.
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References
- Fryer LG, Jones B, Duncan EJ. The endoplasmic reticulum coat protein II transport machinery coordinates cellular lipid secretion and cholesterol biosynthesis. The Journal of biological chemistry. 289(7):4244-61. 2014.
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