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Product Name
SDHA Polyclonal Antibody
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Description
Polyclonal antibody to SDHA
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
SDHA antibody; CMD1GG antibody; FP antibody; PGL5 antibody; SDH1 antibody; SDH2 antibody; SDHF antibody; succinate dehydrogenase complex flavoprotein subunit A antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 385-664 of human SDHA (NP_004159.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100 -
Validations
Western blot - SDHA Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SDHA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.
Immunohistochemistry - SDHA Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat heart using SDHA antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SDHA Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver cancer using SDHA antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SDHA Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human esophageal cancer using SDHA antibody at dilution of 1:100 (40x lens).
Immunofluorescence - SDHA Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using SDHA antibody . Blue: DAPI for nuclear staining.
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Background
Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"