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Product Name
Anti-SDHA Rabbit antibody
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Description
SDHA Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
FP; PGL5; SDH1; SDH2; SDHF; CMD1GG antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human SDHA
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000
IHC: 1/20-1/100
ICC/IF: 1/50
FC: 1/20
IP: 1/20
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Validations
Immunohistochemistry of SDHA in paraffin-embedded Human colon cancer tissue using SDHA Rabbit pAb at dilution 1/20
Western blot detection of SDHA in K562,C6,3T3 cell lysates using SDHA Rabbit pAb(1:1000 diluted).Predicted band size:73kDa.Observed band size:73kDa.
Immunocytochemistry of SDHA(green) in Hela cells using SDHA Rabbit pAb at dilution 1/50, and DAPI(blue)
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Background
Swiss-Prot Acc.P31040.Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (PubMed:24781757). Can act as a tumor suppressor (PubMed:20484225).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"