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Product Name
SARS Polyclonal Antibody
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Description
Polyclonal antibody to SARS
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
SARS antibody; SERRS antibody; SERS antibody; seryl-tRNA synthetase antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SARS (NP_006504.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - SARS Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SARS antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - SARS Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat spinal cord using SARS Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SARS Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using SARS Antibody at dilution of 1:100 (40x lens).
Immunofluorescence - SARS Polyclonal Antibody
Immunofluorescence analysis of MCF7 cells using SARS antibody . Blue: DAPI for nuclear staining.
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Background
Catalyzes the attachment of serine to tRNA(Ser) in a two-step reaction: serine is first activated by ATP to form Ser-AMP and then transferred to the acceptor end of tRNA(Ser). Is probably also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec). In the nucleus, binds to the VEGFA core promoter and prevents MYC binding and transcriptional activation by MYC. Recruits SIRT2 to the VEGFA promoter, promoting deacetylation of histone H4 at 'Lys-16' (H4K16). Thereby, inhibits the production of VEGFA and sprouting angiogenesis mediated by VEGFA.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"