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Product Name
SARS antibody
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Description
SARS Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, HeLa cells, HepG2 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human lung cancer tissue. Observed molecular weight by Western-blot: 58-64 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ36399 antibody; SARS antibody; Serine tRNA ligase antibody; SERRS antibody; SERS antibody; seryl tRNA synthetase antibody; Seryl tRNA(Ser/Sec) synthetase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SARS recombinant protein (Accession Number: NM_006513). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:114970(SARS antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:114970(SARS antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:114970(SARS antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:114970(SARS Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
SARS(Serine--tRNA ligase, cytoplasmic) Catalyzes the attachment of serine to tRNA(Ser). Is also probably able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).The deduced 514-amino acid protein has a calculated molecular mass of about 59 kD.And it can form a dimer of 120KDa(PMID:2180700).It is detected a band of 65KDa in MEL cell lines(PMID:1918033).
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