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Sam68 antibody

Cat.#: 115049

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Product Information

  • Product Name
    Sam68 antibody
  • Documents
  • Description
    Sam68 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HEK-293 cells, mouse brain tissue, NIH/3T3 cells. Positive IP detected in HeLa cells. Positive FC detected in MCF-7 cells. Positive IHC detected in human breast cancer tissue. Observed molecular weight by Western-blot: 68 kDa
  • Tested applications
    ELISA, WB, IHC, FC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    FLJ34027 antibody; KHDRBS1 antibody; p62 antibody; p68 antibody; Sam68 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Sam68 recombinant protein (Accession Number: NM_006559). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115049(KHDRBS1 antibody) at dilution of 1:1000

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115049(KHDRBS1 antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:115049(KHDRBS1 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:115049(KHDRBS1 antibody) at dilution of 1:50 (under 10x lens)

    IP Result of anti-Sam68 (IP:Catalog No:115049, 4ug; Detection:Catalog No:115049 1:1000) with HeLa cells lysate 1200ug.

    IP Result of anti-Sam68 (IP:Catalog No:115049, 4ug; Detection:Catalog No:115049 1:1000) with HeLa cells lysate 1200ug.

    1X10^6 MCF-7 cells were stained with 0.2ug Sam68 antibody (Catalog No:115049, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 MCF-7 cells were stained with 0.2ug Sam68 antibody (Catalog No:115049, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    KHDRBS1, also named as SAM68, p62and p68, belongs to the KHDRBS family. It is recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, KHDRBS1 functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. It represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. KHDRBS1 also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export. KHDRBS1 has three isoforms with calculated MW of 44-48kd. For isoform with a calculated MW of 48.2 kD, it migrated as a 68kD protein on SDS-PAGE gels[PMID: 10564820]. This ia a rabbit polyclonal antibody raised against residues near the N terminus of human KHDRBS1.
  • References
    • Neumann M, Valori CF, Ansorge O. Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations. Acta neuropathologica. 124(5):705-16. 2012.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"