-
Product Name
SAG Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to SAG
-
Tested applications
WB, IHC
-
Species reactivity
Mouse, Rat
-
Alternative names
SAG antibody; RP47 antibody; S-AG antibody; S-arrestin antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-405 of human SAG (NP_000532.2).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:1000 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - SAG Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SAG antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - SAG Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse retina using SAG antibody at dilution of 1:200 (40x lens).
-
Background
Binds to photoactivated, phosphorylated RHO and terminates RHO signaling via G-proteins by competing with G-proteins for the same binding site on RHO (By similarity). May play a role in preventing light-dependent degeneration of retinal photoreceptor cells.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"