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Product Name
S100A16 antibody
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Description
S100A16 Rabbit Polyclonal antibody. Positive WB detected in A431 cells, Transfected HEK-293 cells. Positive IHC detected in human lung cancer tissue, human colon cancer tissue. Positive IF detected in MCF-7 cells. Observed molecular weight by Western-blot: 9-12 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
AAG13 antibody; DT1P1A7 antibody; Protein S100 A16 antibody; Protein S100 F antibody; S100A16 antibody; S100F antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of S100A16 recombinant protein (Accession Number: NM_080388). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:50-1:500
IF: 1:50-1:500
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Validations
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:114959(S100A16 Antibody) at dilution of 1:1000
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:114959(S100A16 Antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using Catalog No:114959(S100A16 Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using Catalog No:114959(S100A16 Antibody) at dilution of 1:200 (under 40x lens).
Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using Catalog No:114959(S100A16 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
S100A16 represents S100 calcium binding protein A16
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References
- Zhang R, Zhu W, Du X. S100A16 mediation of weight gain attenuation induced by dietary calcium. Metabolism: clinical and experimental. 61(2):157-63. 2012.
- Sapkota D, Costea DE, Ibrahim SO, Johannessen AC, Bruland O. S100A14 interacts with S100A16 and regulates its expression in human cancer cells. PloS one. 8(9):e76058. 2013.
- Sapkota D, Bruland O, Parajuli H. S100A16 promotes differentiation and contributes to a less aggressive tumor phenotype in oral squamous cell carcinoma. BMC cancer. 15:631. 2015.
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