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Product Name
S100A14 antibody
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Description
S100A14 Rabbit Polyclonal antibody. Positive IHC detected in human skin tissue, human colon cancer tissue, human gliomas tissue. Positive IF detected in HeLa cells.
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Tested applications
ELISA, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
Protein S100 A14 antibody; S100A14 antibody; S100A15 antibody; S114 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of S100A14 recombinant protein (Accession Number: XM_017001875). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Immunohistochemical of paraffin-embedded human skin using Catalog No:114958(S100A14 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human skin using Catalog No:114958(S100A14 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:114958(S100A14 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
The S100 family is a multifunctional group of EF-hand type calciumbinding proteins. S100A14 protein (previously known as BCMP84, S100A15) is a recently identified member of the S100 family. It is differentially expressed in a number of different human malignancies like ovary, breast, uterus, kidney, rectum and colon cancers, and esophageal and oral squamous cell carcinomas (OSCCs).
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References
- Sapkota D, Bruland O, Costea DE, Haugen H, Vasstrand EN, Ibrahim SO. S100A14 regulates the invasive potential of oral squamous cell carcinoma derived cell-lines in vitro by modulating expression of matrix metalloproteinases, MMP1 and MMP9. European journal of cancer (Oxford, England : 1990). 47(4):600-10. 2011.
- Sapkota D, Costea DE, Blø M. S100A14 inhibits proliferation of oral carcinoma derived cells through G1-arrest. Oral oncology. 48(3):219-25. 2012.
- Leth-Larsen R, Terp MG, Christensen AG. Functional heterogeneity within the CD44 high human breast cancer stem cell-like compartment reveals a gene signature predictive of distant metastasis. Molecular medicine (Cambridge, Mass.). 18:1109-21. 2012.
- Ko CH, Cheng CF, Lai CP. Differential proteomic analysis of cancer stem cell properties in hepatocellular carcinomas by isobaric tag labeling and mass spectrometry. Journal of proteome research. 12(8):3573-85. 2013.
- Sapkota D, Costea DE, Ibrahim SO, Johannessen AC, Bruland O. S100A14 interacts with S100A16 and regulates its expression in human cancer cells. PloS one. 8(9):e76058. 2013.
- Lunde ML, Roman E, Warnakulasuriya S. Profiling of chromosomal changes in potentially malignant and malignant oral mucosal lesions from South and South-East Asia using array-comparative genomic hybridization. Cancer genomics & proteomics. 11(3):127-40. 2014.
- Cho H, Shin HY, Kim S. The role of S100A14 in epithelial ovarian tumors. Oncotarget. 5(11):3482-96. 2014.
- Zhang Q, Zhu M, Cheng W. Downregulation of 425G>a variant of calcium-binding protein S100A14 associated with poor differentiation and prognosis in gastric cancer. Journal of cancer research and clinical oncology. 141(4):691-703. 2015.
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