-
Product Name
RUFY1 antibody
- Documents
-
Description
RUFY1 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 69kd and 80k
-
Tested applications
ELISA, WB, IF, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
FLJ22251 antibody; FYVE finger protein EIP1 antibody; La binding protein 1 antibody; Rab4 interacting protein antibody; RABIP4 antibody; RUFY1 antibody; ZFYVE12 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of RUFY1 recombinant protein (Accession Number: NM_001040452). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:1000
IF: 1:20-1:200
-
Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114868(RUFY1 antibody) at dilution of 1:1000
IP result of anti-RUFY1(Catalog No:114868 for IP and Detection).
Immunofluorescent analysis of Hela cells, using RUFY1 antibody Catalog No:114868 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
-
Background
Rab proteins form the largest group within the Ras superfamily of small GTPases, which acts as molecular switches by cycling between their inactive active GTP–bound and GDP–bound forms [PMID:15520808]. The small GTP-binding protein Rab4 has been involved in the recycling of αvβ3 integrins in response to platelet-derived growth factor (PDGF) stimulation suggesting a role for Rab4 in cell adhesion and migration [PMID:17001082]. Rabip4 (Rab4 interacting protein), which possesses a RUN domain, two coiled-coil domains and a FYVE finger, was identified as an effector of Rab4, a small GTPase that plays a role in the endocytotic pathway in all cell types [PMID:11172003].
-
References
- Gomez TS, Gorman JA, de Narvajas AA, Koenig AO, Billadeau DD. Trafficking defects in WASH-knockout fibroblasts originate from collapsed endosomal and lysosomal networks. Molecular biology of the cell. 23(16):3215-28. 2012.
- Phillips-Krawczak CA, Singla A, Starokadomskyy P. COMMD1 is linked to the WASH complex and regulates endosomal trafficking of the copper transporter ATP7A. Molecular biology of the cell. 26(1):91-103. 2015.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"