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Product Name
RRAGC Polyclonal Antibody
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Description
Polyclonal antibody to RRAGC
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
RRAGC antibody; GTR2 antibody; RAGC antibody; TIB929 antibody; Ras related GTP binding C antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human RRAGC (NP_071440.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - RRAGC Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using RRAGC antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 30s.
Immunohistochemistry - RRAGC Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat intestine using RRAGC Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - RRAGC Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using RRAGC Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - RRAGC Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse intestine using RRAGC Antibody at dilution of 1:100 (40x lens).
Immunofluorescence - RRAGC Polyclonal Antibody
Immunofluorescence analysis of A549 cells using RRAGC antibody .
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Background
Guanine nucleotide-binding protein forming heterodimeric Rag complexes required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"