RRAGC Polyclonal Antibody

Cat.#: 165392

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Product Information

  • Product Name
    RRAGC Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to RRAGC
  • Tested applications
    WB, IHC, IF
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    RRAGC antibody; GTR2 antibody; RAGC antibody; TIB929 antibody; Ras related GTP binding C antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human RRAGC (NP_071440.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:200
    IF 1:50 - 1:200
  • Validations

    Western blot - RRAGC Polyclonal Antibody

    Western blot - RRAGC Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using RRAGC antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 30s.

    Immunohistochemistry - RRAGC Polyclonal Antibody

    Immunohistochemistry - RRAGC Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded rat intestine using RRAGC Antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - RRAGC Polyclonal Antibody

    Immunohistochemistry - RRAGC Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded rat brain using RRAGC Antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - RRAGC Polyclonal Antibody

    Immunohistochemistry - RRAGC Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded mouse intestine using RRAGC Antibody at dilution of 1:100 (40x lens).

    Immunofluorescence - RRAGC Polyclonal Antibody

    Immunofluorescence - RRAGC Polyclonal Antibody

    Immunofluorescence analysis of A549 cells using RRAGC antibody .

  • Background
    Guanine nucleotide-binding protein forming heterodimeric Rag complexes required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"