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Product Name
RPS27A antibody
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Description
RPS27A Rabbit Polyclonal antibody. Positive WB detected in mouse cerebellum tissue, Jurkat cells. Positive IHC detected in human endometrial cancer tissue, human cerebellum tissue. Observed molecular weight by Western-blot: 9 kDa, 18 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CEP80 antibody; HUBCEP80 antibody; ribosomal protein S27a antibody; RPS27A antibody; UBA80 antibody; UBCEP1 antibody; UBCEP80 antibody; ubiquitin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RPS27A recombinant protein (Accession Number: NM_001177413). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
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Validations
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with Catalog No:114839(RPS27A antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human cerebellum tissue slide using Catalog No:114839(RPS27A Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human endometrial cancer tissue slide using Catalog No:114839(RPS27A Antibody) at dilution of 1:50 (under 40x lens)
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Background
RPS27A, also named as UBA80 and UBCEP1, can be cleaved into two chains Ubiquitin and 40S ribosomal protein S27a. Ubiquitin is a highly conserved 76-amino acid protein that plays a key role in protein degradation. Ubiquitin is synthesized as precursor proteins that consist either of polyubiquitin chains that are cleaved into moieties of the UBB or UBC types, or single ubiquitin moieties fused 5-prime to unrelated carboxyl extension proteins (UBA type) such as UBA52 and UBA80 (RPS27A) [PMID:10772958]
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References
- Tian Y, Ding W, Wang Y. Ubiquitin B in cervical cancer: critical for the maintenance of cancer stem-like cell characters. PloS one. 8(12):e84457. 2013.
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