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Product Name
RPS12 antibody
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Description
RPS12 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, MCF7 cells, NIH/3T3 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 15kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
40S ribosomal protein S12 antibody; ribosomal protein S12 antibody; RPS12 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RPS12 recombinant protein (Accession Number: NM_001016). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114827(RPS12 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:114827(RPS12 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:114827(RPS12 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using RPS12 antibody Catalog No:114827 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
RPS12 (ribosomal protein S12) is a plastid ribosomal protein which is a part of the 30S ribosomal subunit. Together with S4 and S5 plays an important role in translational accuracy.This antibody specifically recognizes the 15kd RPS12 protein.
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References
- Chen D, Zhang R, Shen W. RPS12-specific shRNA inhibits the proliferation, migration of BGC823 gastric cancer cells with S100A4 as a downstream effector. International journal of oncology. 42(5):1763-9. 2013.
- Raines AM, Adam M, Magella B. Recombineering-based dissection of flanking and paralogous Hox gene functions in mouse reproductive tracts. Development (Cambridge, England). 140(14):2942-52. 2013.
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