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Product Name
RPL7A antibody
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Description
RPL7A Rabbit Polyclonal antibody. Positive IP detected in mouse kidney tissue. Positive WB detected in MCF7 cells, mouse kidney tissue, mouse liver tissue, rat kidney tissue. Positive IHC detected in human breast cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 30-32 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
60S ribosomal protein L7a antibody; PLA X polypeptide antibody; ribosomal protein L7a antibody; RPL7A antibody; SURF 3 antibody; SURF3 antibody; Surfeit lOCus protein 3 antibody; TRUP antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RPL7A recombinant protein (Accession Number: NM_000972). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:114900(RPL7A antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:114900(RPL7A antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:114900(RPL7A antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using RPL7A antibody Catalog No:114900 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-RPL7A (IP:Catalog No:114900, 4ug; Detection:Catalog No:114900 1:1000) with mouse kidney tissue lysate 4000ug.
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Background
Ribosomal proteins are a major component of ribosomes, which catalyze protein synthesis. RPL7a, which is a component of the 60S large ribosomal subunit, has additional functions involved in cell growth and differentiation that occur via interaction with human thyroid hormone receptor (THR) and retinoic acid receptor (RAR) and in turn inhibit the activities of the two nuclear hormone receptors [PMID:21505254].
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References
- Mino T, Murakawa Y, Fukao A. Regnase-1 and Roquin Regulate a Common Element in Inflammatory mRNAs by Spatiotemporally Distinct Mechanisms. Cell. 161(5):1058-73. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"