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Product Name
RPL31 antibody
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Description
RPL31 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, mouse liver tissue, NIH/3T3 cells. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 14 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
60S ribosomal protein L31 antibody; ribosomal protein L31 antibody; RPL31 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RPL31 recombinant protein (Accession Number: NM_000993). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:50-1:500
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:114889(RPL31 antibody) at dilution of 1:300
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:114889(RPL31 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. Ribosomal protein L31 gene is a component of the 60S large ribosomal subunit encoded by RPL31 gene, while ribosomal protein L31 (RPL31) is an important constituent of peptidyltransferase center [PMID:22714919].
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