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Product Name
RPL14 antibody
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Description
RPL14 Rabbit Polyclonal antibody. Positive IP detected in mouse liver tissue. Positive WB detected in HeLa cells, MCF7 cells, mouse liver tissue, rat liver tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 23 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
60S ribosomal protein L14 antibody; CAG ISL 7 antibody; CTG B33 antibody; hRL14 antibody; L14 antibody; ribosomal protein L14 antibody; RL14 antibody; RPL14 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RPL14 recombinant protein (Accession Number: NM_001034996). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114815(RPL14 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:114815(RPL14 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:114815(RPL14 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using RPL14 antibody Catalog No:114815 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-RPL14 (IP:Catalog No:114815, 4ug; Detection:Catalog No:114815 1:1000) with mouse liver tissue lysate 6800ug.
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Background
RPL14 represents ribosomal protein L14
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References
- Shi Y, Liu X, Li R. Retention of OsNMD3 in the cytoplasm disturbs protein synthesis efficiency and affects plant development in rice. Journal of experimental botany. 65(12):3055-69. 2014.
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