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Product Name
RPB5 antibody
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Description
RPB5 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue. Positive IP detected in HeLa cells. Positive WB detected in Jurkat cells, A549 cells, HeLa cells, HepG2 cells, MCF7 cells, mouse liver tissue, mouse spleen tissue, rat liver tissue, rat spleen tissue. Observed molecular weight by Western-blot: 23-25 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
hRPB25 antibody; hsRPB5 antibody; POLR2E antibody; RPABC1 antibody; RPB5 antibody; RPB5 homolog antibody; XAP4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RPB5 recombinant protein (Accession Number: NM_002695). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:114800(POLR2E antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:114800(POLR2E antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:114800(POLR2E antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-RNA Polymerase II (IP:Catalog No:114800, 4ug; Detection:Catalog No:114800 1:500) with HeLa cells lysate 880ug.
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Background
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. polymerase (RNA) II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E (Polymerase (RNA) II (DNA directed) polypeptide E) /RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process. The observed molecular weight of POLR2E is about 25 kDa.
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References
- Zhang J, Pan YF, Ding ZW. RMP promotes venous metastases of hepatocellular carcinoma through promoting IL-6 transcription. Oncogene. 34(12):1575-83. 2015.
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