RORB antibody

Cat.#: 114789

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Product Information

  • Product Name
    RORB antibody
  • Documents
  • Description
    RORB Rabbit Polyclonal antibody. Positive IHC detected in mouse brain tissue. Positive IP detected in mouse brain tissue. Positive WB detected in Y79 cells, mouse brain tissue, SH-SY5Y cells. Observed molecular weight by Western-blot: 52 kDa
  • Tested applications
    ELISA, WB, IHC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    bA133M9.1 antibody; NR1F2 antibody; Nuclear receptor ROR beta antibody; Nuclear receptor RZR beta antibody; RAR related orphan receptor B antibody; ROR BETA antibody; RORB antibody; RZR BETA antibody; RZRB antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of RORB recombinant protein (Accession Number: NM_006914). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:1000

    IHC: 1:20-1:200

  • Validations

    Y79 cells were subjected to SDS PAGE followed by western blot with Catalog No:114789(RORB antibody) at dilution of 1:800

    Y79 cells were subjected to SDS PAGE followed by western blot with Catalog No:114789(RORB antibody) at dilution of 1:800

    IP Result of anti-RORB (IP:Catalog No:114789, 4ug; Detection:Catalog No:114789 1:300) with mouse brain tissue lysate 3440ug.

    IP Result of anti-RORB (IP:Catalog No:114789, 4ug; Detection:Catalog No:114789 1:300) with mouse brain tissue lysate 3440ug.

    Immunohistochemistry of paraffin-embedded mouse brain tissue slide using Catalog No:114789(RORB Antibody) at dilution of 1:200 (under 40x lens)

    Immunohistochemistry of paraffin-embedded mouse brain tissue slide using Catalog No:114789(RORB Antibody) at dilution of 1:200 (under 40x lens)

  • Background
    Nuclear receptors (NRs) are a large group of ligand-inducible transcription factors that regulate many facets of mammalian Physiology [PMID:22189870]. In addition, NRs that lack identified ligands and are termed orphan receptors. RORB is a transcription factor and belongs to the nuclear receptor family [PMID:7935491], which share a common modular structure composed of a transactivation domain, a DNA-binding domain, and a ligand-binding. Its function as transcriptional factor is regulated by small lipophilic molecules, such as steroid hormones, vitamin D, retinoic acids, and thyroid hormone [PMID:17431400].

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"