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Product Name
ROM1 antibody
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Description
ROM1 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive WB detected in mouse eye tissue, HeLa cells. Observed molecular weight by Western-blot: 37-45kd
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Tested applications
ELISA, WB, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
ROM antibody; ROM1 antibody; ROSP1 antibody; Tetraspanin 23 antibody; Tspan 23 antibody; TSPAN23 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ROM1 recombinant protein (Accession Number: NM_000327). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:20-1:200
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Validations
mouse eye tissue were subjected to SDS PAGE followed by western blot with Catalog No:114783(ROM1 antibody) at dilution of 1:400
Immunofluorescent analysis of HeLa cells using Catalog No:114783(ROM1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
ROM1 (also known as TSPAN23) is a transmembrane protein present in photoreceptor outer segment disc membranes. ROM1 belongs to the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. ROM1 is required for rod photoreceptor viability and the regulation of disk morphogenesis (PMID: 10802659). It may function as an adhesion molecule involved in stabilization and compaction of outer segment disks.
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References
- Datta P, Allamargot C, Hudson JS. Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome. Proceedings of the National Academy of Sciences of the United States of America. 112(32):E4400-9. 2015.
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