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Product Name
RNGTT antibody
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Description
RNGTT Rabbit Polyclonal antibody. Positive WB detected in human kidney tissue. Positive IP detected in mouse kidney tissue. Observed molecular weight by Western-blot: 69 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CAP1A antibody; DKFZp686J2031 antibody; GTase antibody; GTP RNA guanylyltransferase antibody; hCAP antibody; HCAP1 antibody; HCE antibody; HCE1 antibody; mRNA 5 triphosphatase antibody; mRNA capping enzyme antibody; RNGTT antibody; TPase mRNA guanylyltransferase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RNGTT recombinant protein (Accession Number: NM_003800). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
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Validations
human kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:114766(RNGTT antibody) at dilution of 1:800
IP Result of anti-RNGTT (IP:Catalog No:114766, 4ug; Detection:Catalog No:114766 1:500) with mouse kidney tissue lysate 4000ug.
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Background
RNGTT(mRNA-capping enzyme) is also named as HCAP1, HCE, CAP1A. In the N-terminal section it belongs to the non-receptor class of the protein-tyrosine phosphatase family and in the C-terminal section it belongs to the eukaryotic GTase family. It is a bifunctional 597-amino acid protein with RNA triphosphatase and RNA guanylyltransferase activities(PMID:9545288). It has 4 isoforms produced by alternative splicing.
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