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Product Name
Anti-HCE Rabbit antibody
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Description
HCE Rabbit monoclonal antibody
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Tested applications
WB, ICC/IF, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
HCE; HCE1; hCAP; CAP1A antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human HCE
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Clonality
Monoclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
ICC/IF: 1/50
IP: 1/20
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Validations
Western blot detection of HCE in K562,C6,3T3 cell lysates using HCE Rabbit mAb(1:1000 diluted).Predicted band size:69kDa.Observed band size:69kDa.
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Background
Swiss-Prot Acc.O60942.Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"