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Product Name
RNF7 antibody
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Description
RNF7 Rabbit Polyclonal antibody. Positive IP detected in mouse heart tissue. Positive WB detected in human liver tissue, human heart tissue, human skeletal muscle tissue, human testis tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 13 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CKBBP1 antibody; CKII beta binding protein 1 antibody; Rbx2 antibody; Regulator of cullins 2 antibody; RING box protein 2 antibody; ring finger protein 7 antibody; RNF7 antibody; ROC2 antibody; SAG antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RNF7 recombinant protein (Accession Number: NM_014245). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:114763(SAG antibody) at dilution of 1:500
Immunofluorescent analysis of HepG2 cells, using RNF7 antibody Catalog No:114763 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-SAG (IP:Catalog No:114763, 4ug; Detection:Catalog No:114763 1:600) with mouse heart tissue lysate 4400ug.
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Background
RNF (ring finger protein 7 ) is a novel zinc RING finger protein which is redox responsive and protects mammalian cells from apoptosis.It is shown to be localised in the cytoplasmand nucleus of the cell and is expressed in the heart, liver, skeletal muscle predominantly. RNF7 forms oligomers(55 kDa),dimer(28 kDa) and monomer(13 kDa) in the presence of different concentrations of the reductant.
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