-
Product Name
RNF38 antibody
- Documents
-
Description
RNF38 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human endometrial cancer tissue. Observed molecular weight by Western-blot: 43-58 kDa
-
Tested applications
ELISA, WB, IHC, IF
-
Species reactivity
Human; other species not tested.
-
Alternative names
FLJ21343 antibody; ring finger protein 38 antibody; RNF38 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of RNF38 recombinant protein (Accession Number: NM_194329). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:50-1:500
-
Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:114758(RNF38 Antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human endometrial cancer tissue slide using Catalog No:114758(RNF38 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunohistochemistry of paraffin-embedded human endometrial cancer tissue slide using Catalog No:114758(RNF38 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:114758(RNF38 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
RING finger protein 38 is a protein that in humans is encoded by the RNF38 gene.This gene encodes a protein with a coiled-coil motif and a RING-H2 motif (C3H2C2) at its carboxy-terminus. The RING motif is a zinc-binding domain found in a large set of proteins playing roles in diverse cellular processes including oncogenesis, development, signal transduction, and apoptosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. RNF38 is widely expressed with highest levels in testis.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"