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Product Name
RNase T2 antibody
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Description
RNase T2 Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, mouse pancreas tissue. Positive IP detected in BxPC-3 cells. Positive IHC detected in human pancreas cancer tissue. Positive IF detected in transfected cells. Observed molecular weight by Western-blot: 29 kDa
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Tested applications
ELISA, IHC, IF, WB, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
bA514O12.3 antibody; Ribonuclease 6 antibody; Ribonuclease T2 antibody; RNASE6PL antibody; RNASET2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RNase T2 recombinant protein (Accession Number: NM_003730). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:114725(RNASET2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:114725(RNASET2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:114725(RNASET2 antibody) at dilution of 1:100 (under 40x lens)
IF result of Catalog No:114725(anti-RNASET2) in COS7 cell by Dr. Nancy Kedersha. COS7 cells transiently transfected with FAST-KD4-YFP, counterstained with PTG RNASET2 antibody (red) and human anti-mitochondrial antigen in blue. Note that FAST-KD4-YFP overexpression alters mitchonrial morphology, and is also present at stress granules (green only).
IP Result of anti-RNASET2 (IP:Catalog No:114725, 4ug; Detection:Catalog No:114725 1:300) with BxPC-3 cells lysate 4000ug.
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Background
RNASET2(Ribonuclease T2) is also named as RNASE6PL(Ribonuclease 6) and belongs to the RNase T2 family. It is a unique member of the growing family of tumor-antagonizing genes and behaves as a class II tumor suppressor and abolish the tumorigenic potential of an ovarian cancer-derived cell line. This protein has 2 isoforms produced by alternative splicing. Both mildly glycosylated (ranging from38 to 45 kDa) and highly glycosylated forms (ranging from 50 to 80 kDa) of human tumor suppressor protein RNASET2 can be detected in immunoblotting in P. Pastoris(PMID: 21446958).
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