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Product Name
RLIM antibody
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Description
RLIM Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, Jurkat cells. Observed molecular weight by Western-blot: 75 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp686N06224 antibody; FLJ25923 antibody; FLJ41093 antibody; FLJ42887 antibody; FLJ45986 antibody; NY REN 43 antibody; R LIM antibody; RING finger protein 12 antibody; RLIM antibody; RNF12 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RLIM recombinant protein (Accession Number: NM_183353). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114721(RLIM antibody) at dilution of 1:500
IP Result of anti-RLIM (IP:Catalog No:114721, 4ug; Detection:Catalog No:114721 1:500) with HeLa cells lysate 1520ug.
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Background
RLIM represents ring finger protein, LIM domain interacting
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References
- Chelmicki T, Dündar F, Turley MJ. MOF-associated complexes ensure stem cell identity and Xist repression. eLife. 3:e02024. 2014.
- Li C, Zhou X, Chen J. PIWIL1 destabilizes microtubule by suppressing phosphorylation at Ser16 and RLIM-mediated degradation of Stathmin1. Oncotarget. 6(29):27794-804. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"