RIPK1-Specific Rabbit Polyclonal antibody. Positive WB detected in NIH/3T3 cells, HeLa cells, K-562 cells, rat liver tissue, UV treated Hela cells. Positive IP detected in HeLa cells. Positive IF detected in HepG2 cells. Positive IHC detected in human heart tissue. Observed molecular weight by Western-blot: 74 kDa
ELISA, WB, IF, IP, IHC
Human,Mouse,Rat; other species not tested.
Cell death protein RIP antibody; FLJ39204 antibody; Receptor interacting protein 1 antibody; RIP antibody; RIP 1 antibody; RIP1 antibody; RIPK1 antibody
This antibody was obtained by immunization of Peptide (Accession Number: NM_003804). Purification method: Antigen affinity purified.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:114715(RIPK1-Specific antibody) at dilution of 1:300
IP Result of anti-RIPK1-Specific (IP:Catalog No:114715, 4ug; Detection:Catalog No:114715 1:300) with HeLa cells lysate 2000ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:114715(RIPK1-Specific Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:114715(RIPK1-Specific Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:114715(RIPK1-Specific Antibody) at dilution of 1:50 (under 40x lens)
RIPK1(Receptor-interacting serine/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis and necroptosis and belongs to a novel class of kinases thatfunction in cell survival and cell death mechanisms(PMID:22685397 ).It has 2 isoforms produced by alternative splicing.It also can exist as the protein with lower molecular weight about 25kd and 45kd in HAEC and HUVEC(PMID:22685397).
- Wang HJ, He WQ, Chen L. Type I collagen gel protects murine fibrosarcoma L929 cells from TNFα-induced cell death. Biochemical and biophysical research communications. 457(4):693-9. 2015.
- Nie D, Zhang D, Dai J. Nicotine Induced Murine Spermatozoa Apoptosis via Up-Regulation of Deubiquitinated RIP1 by Trim27 Promoter Hypomethylation. Biology of reproduction. 94(2):31. 2016.
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