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Product Name
RIPK1-Specific antibody
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Description
RIPK1-Specific Rabbit Polyclonal antibody. Positive WB detected in NIH/3T3 cells, HeLa cells, K-562 cells, rat liver tissue, UV treated Hela cells. Positive IP detected in HeLa cells. Positive IF detected in HepG2 cells. Positive IHC detected in human heart tissue. Observed molecular weight by Western-blot: 74 kDa
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Tested applications
ELISA, WB, IF, IP, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Cell death protein RIP antibody; FLJ39204 antibody; Receptor interacting protein 1 antibody; RIP antibody; RIP 1 antibody; RIP1 antibody; RIPK1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_003804). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:114715(RIPK1-Specific antibody) at dilution of 1:300
IP Result of anti-RIPK1-Specific (IP:Catalog No:114715, 4ug; Detection:Catalog No:114715 1:300) with HeLa cells lysate 2000ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:114715(RIPK1-Specific Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:114715(RIPK1-Specific Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:114715(RIPK1-Specific Antibody) at dilution of 1:50 (under 40x lens)
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Background
RIPK1(Receptor-interacting serine/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis and necroptosis and belongs to a novel class of kinases thatfunction in cell survival and cell death mechanisms(PMID:22685397 ).It has 2 isoforms produced by alternative splicing.It also can exist as the protein with lower molecular weight about 25kd and 45kd in HAEC and HUVEC(PMID:22685397).
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References
- Wang HJ, He WQ, Chen L. Type I collagen gel protects murine fibrosarcoma L929 cells from TNFα-induced cell death. Biochemical and biophysical research communications. 457(4):693-9. 2015.
- Nie D, Zhang D, Dai J. Nicotine Induced Murine Spermatozoa Apoptosis via Up-Regulation of Deubiquitinated RIP1 by Trim27 Promoter Hypomethylation. Biology of reproduction. 94(2):31. 2016.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"