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Product Name
ribosomal protein L4 antibody
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Description
ribosomal protein L4 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human lymphoma tissue. Positive IP detected in K-562 cells. Positive WB detected in rat brain tissue, A375 cells, HeLa cells, human skeletal muscle tissue, K-562 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue, U-937 cells. Observed molecular weight by Western-blot: 48kd
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Tested applications
ELISA, IHC, IF, IP, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
60S ribosomal protein L1 antibody; 60S ribosomal protein L4 antibody; ribosomal protein L4 antibody; RPL1 antibody; RPL4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ribosomal protein L4 recombinant protein (Accession Number: NM_000968). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
rat brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114696(RPL4 Antibody) at dilution of 1:1000
IP Result of anti-RPL4 (IP:Catalog No:114696, 3ug; Detection:Catalog No:114696 1:800) with K-562 cells lysate 6000ug.
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:114696(RPL4 antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using RPL4 antibody Catalog No:114696 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.
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References
- Rosado A, Sohn EJ, Drakakaki G. Auxin-mediated ribosomal biogenesis regulates vacuolar trafficking in Arabidopsis. The Plant cell. 22(1):143-58. 2010.
- Liang XH, Vickers TA, Guo S, Crooke ST. Efficient and specific knockdown of small non-coding RNAs in mammalian cells and in mice. Nucleic acids research. 39(3):e13. 2011.
- Bartoli KM, Jakovljevic J, Woolford JL, Saunders WS. Kinesin molecular motor Eg5 functions during polypeptide synthesis. Molecular biology of the cell. 22(18):3420-30. 2011.
- Liang XH, Crooke ST. RNA helicase A is not required for RISC activity. Biochimica et biophysica acta. 1829(10):1092-101. 2013.
- Shi Y, Liu X, Li R. Retention of OsNMD3 in the cytoplasm disturbs protein synthesis efficiency and affects plant development in rice. Journal of experimental botany. 65(12):3055-69. 2014.
- Zhang X, Gao X, Coots RA, Conn CS, Liu B, Qian SB. Translational control of the cytosolic stress response by mitochondrial ribosomal protein L18. Nature structural & molecular biology. 22(5):404-10. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"