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Product Name
RIAM antibody
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Description
RIAM Rabbit Polyclonal antibody. Positive IHC detected in human lymphoma tissue. Positive WB detected in Jurkat cells. Positive IP detected in Jurkat cells. Observed molecular weight by Western-blot: 100kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
APBB1 interacting protein 1 antibody; APBB1IP antibody; INAG1 antibody; PREL 1 antibody; PREL1 antibody; Proline rich EVH1 ligand 1 antibody; Proline rich protein 73 antibody; RARP 1 antibody; RARP1 antibody; RIAM antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RIAM recombinant protein (Accession Number: BC035636). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:114694(RIAM,APBB1IP antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:114694(RIAM,APBB1IP antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:114694(RIAM,APBB1IP antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-RIAM,APBB1IP (IP:Catalog No:114694, 5ug; Detection:Catalog No:114694 1:500) with Jurkat cells lysate 2400ug.
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Background
APBB1IP gene encodes a Rap1-GTP-interacting adapter molecule (RIAM) which is an important protein in Rap1-mediated integrin activation and adhesion. Rap1 is a small GTPase frequently activated in tumors such as melanoma and prostate cancer. RIAM is widely expressed with high expression in thymus, spleen, lymph node, bone marrow and peripheral leukocytes. RIAM may function in the signal transduction from Ras activation to actin cytoskeletal remodeling. The observed molecular weight of RIAM is about 100 kDa.
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References
- Königsberger S, Peckl-Schmid D, Zaborsky N, Patzak I, Kiefer F, Achatz G. HPK1 associates with SKAP-HOM to negatively regulate Rap1-mediated B-lymphocyte adhesion. PloS one. 5(9):. 2010.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"