-
Product Name
RHOT2 antibody
- Documents
-
Description
RHOT2 Rabbit Polyclonal antibody. Positive IHC detected in human heart tissue, human colon tissue, human kidney tissue, human lung cancer tissue. Positive IP detected in L02 cells. Positive WB detected in human testis tissue, human heart tissue, human kidney tissue, L02 cells, mouse heart tissue. Observed molecular weight by Western-blot: 68 kDa
-
Tested applications
ELISA, IHC, WB, IP
-
Species reactivity
Human, Mouse; other species not tested.
-
Alternative names
ARHT2 antibody; C16orf39 antibody; hMiro 2 antibody; MIRO 2 antibody; MitOChondrial Rho GTPase 2 antibody; RASL antibody; RHOT2 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of RHOT2 recombinant protein (Accession Number: NM_138769). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
-
Validations
human testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:114664(RHOT2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human heart using Catalog No:114664(RHOT2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:114664(RHOT2 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-RHOT2 (IP:Catalog No:114664, 3ug; Detection:Catalog No:114664 1:500) with L02 cells lysate 1560ug.
-
Background
RHOT2 (Ras homolog gene family, member T2), also known as ARHT2, RASL or MIRO-2 (mitochondrial Rho GTPase 2), is a 618 amino acid single-pass type IV membrane protein as a member of the Rho family of GTPases. It is localized to the outer mitochondrial membrane and plays a role in mitochondrial trafficking and fusion-fission dynamics. RHOT2 is ubiquitously expressed with highest levels found in pancreas, heart, kidney and skeletal muscle.
-
References
- Weihofen A, Thomas KJ, Ostaszewski BL, Cookson MR, Selkoe DJ. Pink1 forms a multiprotein complex with Miro and Milton, linking Pink1 function to mitochondrial trafficking. Biochemistry. 48(9):2045-52. 2009.
- Cagalinec M, Safiulina D, Liiv M. Principles of the mitochondrial fusion and fission cycle in neurons. Journal of cell science. 126(Pt 10):2187-97. 2013.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"