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Product Name
RHOC Polyclonal Antibody
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Description
Polyclonal antibody to RHOC
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
RHOC antibody; ARH9 antibody; ARHC antibody; H9 antibody; RHOH9 antibody; ras homolog family member C antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human RHOC (NP_001036144.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100 -
Validations
Western blot - RHOC Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using RHOC antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - RHOC Polyclonal Antibody
Western blot analysis of extracts from normal (control) and RHOC knockout 293T cells, using RHOC antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 300s.CRISPR KO cell lines generated by
Immunofluorescence - RHOC Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using RHOC antibody . Blue: DAPI for nuclear staining.
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Background
Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Regulates apical junction formation in bronchial epithelial cells.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"