RHOC Polyclonal Antibody

Cat.#: 160586

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Product Information

  • Product Name
    RHOC Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to RHOC
  • Tested applications
    WB, IHC, IF
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    RHOC antibody; ARH9 antibody; ARHC antibody; H9 antibody; RHOH9 antibody; ras homolog family member C antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human RHOC (NP_001036144.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:100
    IF 1:50 - 1:100
  • Validations

    Western blot - RHOC Polyclonal Antibody

    Western blot - RHOC Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using RHOC antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

    Western blot - RHOC Polyclonal Antibody

    Western blot - RHOC Polyclonal Antibody

    Western blot analysis of extracts from normal (control) and RHOC knockout 293T cells, using RHOC antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 300s.CRISPR KO cell lines generated by

    Immunofluorescence - RHOC Polyclonal Antibody

    Immunofluorescence - RHOC Polyclonal Antibody

    Immunofluorescence analysis of HeLa cells using RHOC antibody . Blue: DAPI for nuclear staining.

  • Background
    Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Regulates apical junction formation in bronchial epithelial cells.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"