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Product Name
RHOA/RHOB/RHOC Monoclonal Antibody
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Description
Monoclonal antibody to RHOA/RHOB/RHOC
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human RHOC
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:500 - 1:1000
IF 1:100 - 1:500 -
Validations
Western blot - RHOA/RHOB/RHOC Monoclonal Antibody
Western blot analysis of extracts of various cell lines, using RHOC/RHOA/RHOB antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry - RHOA/RHOB/RHOC Monoclonal Antibody
Immunohistochemistry of paraffin-embedded mouse stomach using RHOC/RHOA/RHOB antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - RHOA/RHOB/RHOC Monoclonal Antibody
Immunohistochemistry of paraffin-embedded human gastric cancer using RHOC/RHOA/RHOB antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - RHOA/RHOB/RHOC Monoclonal Antibody
Immunohistochemistry of paraffin-embedded mouse colon using RHOC/RHOA/RHOB antibody at dilution of 1:100 (40x lens).
Immunofluorescence - RHOA/RHOB/RHOC Monoclonal Antibody
Immunofluorescence analysis of NIH-3T3 cells using RHOC/RHOA/RHOB antibody .
Immunofluorescence - RHOA/RHOB/RHOC Monoclonal Antibody
Immunofluorescence analysis of Ags cells using RHOC/RHOA/RHOB antibody .
Immunofluorescence - RHOA/RHOB/RHOC Monoclonal Antibody
Immunofluorescence analysis of HepG2 cells using RHOC/RHOA/RHOB antibody .
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Background
Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Regulates apical junction formation in bronchial epithelial cells.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"